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ProductsBack/FITC Anti-Mouse Foxp3 Antibody [3G3]

FITC Anti-Mouse Foxp3 Antibody [3G3]

AGEL2482
Assay Genie
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityMouse
TargetFoxp3
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Overview

  • Supplier
    Assay Genie
  • Product Name
    FITC Anti-Mouse Foxp3 Antibody [3G3]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry
  • Applications Supplier
    ICFCM
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3G3
  • Conjugate
    FITC
  • Gene ID20371
  • Target name
    Foxp3
  • Target description
    forkhead box P3
  • Target synonyms
    JM2, scurfin, sf, forkhead box protein P3, scurfy
  • Host
    Mouse
  • Protein IDQ99JB6
  • Protein Name
    Forkhead box protein P3
  • Scientific Description
    FITC Anti-Mouse Foxp3 Antibody [3G3] is a high-performance monoclonal that specifically targets Mouse Foxp3, delivering exceptional specificity and reliability for your research applications. Rigorously validated for ICFCM, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Mouse samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100 Tests format to meet your experimental needs. For optimal performance, store at 4°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Mouse
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Foxp3 using anti-Foxp3 antibody (A00011-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Foxp3 antigen affinity purified polyclonal antibody (Catalog # A00011-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Foxp3 at approximately 50 kDa. The expected band size for Foxp3 is at 50 kDa.
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