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FOXP3 antibody [C3], C-term

GTX107737
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetFoxp3
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Overview

  • Supplier
    GeneTex
  • Product Name
    FOXP3 antibody [C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID20371
  • Target name
    Foxp3
  • Target description
    forkhead box P3
  • Target synonyms
    forkhead box protein P3; JM2; scu; scurfin; scurfy; sf
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99JB6
  • Protein Name
    Forkhead box protein P3
  • Scientific Description
    Probable transcription factor. Plays a critical role in the control of immune response.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Adiponectin-expressing Treg-containing T cell fraction inhibits tumor growth in orthotopically implanted triple-negative breast cancer.
    Read more
  • Potential activity of adiponectin-expressing regulatory T cells against triple-negative breast cancer cells through the cell-in-cell phenomenon.
    Read more
  • Adiponectin-expressing Treg cells in experimental thymic tumor model.
    Read more
  • Protein phosphatase 2A inactivation induces microsatellite instability, neoantigen production and immune response. Yen YT et al., 2021 Dec 15, Nat Commun
    Read more
  • PDE9 Inhibitor PF-04447943 Attenuates DSS-Induced Colitis by Suppressing Oxidative Stress, Inflammation, and Regulating T-Cell Polarization. Rana MN et al., 2021, Front Pharmacol
    Read more
  • Modulation of serotonin in the gut-liver neural axis ameliorates the fatty and fibrotic changes in non-alcoholic fatty liver. Ko M et al., 2021 Mar 28, Dis Model Mech
    Read more
  • Cimetidine promotes STUB1-mediated degradation of tumoral FOXP3 by activating PI3K-Akt pathway in gastric cancer. Zhang L et al., 2020 Oct, Ann Transl Med
    Read more
  • Use of Tregs as a cell-based therapy via CD39 for benign prostate hyperplasia with inflammation. Jin X et al., 2020 May, J Cell Mol Med
    Read more
  • Targeting the Kynurenine Pathway for the Treatment of Cisplatin-Resistant Lung Cancer. Nguyen DJM et al., 2020 Jan, Mol Cancer Res
    Read more

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Figure 1. Western blot analysis of Foxp3 using anti-Foxp3 antibody (A00011-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Foxp3 antigen affinity purified polyclonal antibody (Catalog # A00011-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Foxp3 at approximately 50 kDa. The expected band size for Foxp3 is at 50 kDa.
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IHC-P analysis of mouse thymus (Right panel shows staining without primary antibody as negative control) using GTX89752 FOXP3 antibody, C-term. Antigen retrieval : Tris/EDTA buffer pH 9 Dilution : 2microg/ml
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