![FOXP3 antibody [C3], C-term detects FOXP3 protein at cytoplasm and nucleus in mouse spleen by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min FOXP3 antibody [C3], C-term detects FOXP3 protein at cytoplasm and nucleus in mouse spleen by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX107737/GTX107737_39715_20170929_IHC-P_M_w_23060120_374.webp)
FOXP3 antibody [C3], C-term detects FOXP3 protein at cytoplasm and nucleus in mouse spleen by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
FOXP3 antibody [C3], C-term
GTX107737
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetFoxp3
Overview
- SupplierGeneTex
- Product NameFOXP3 antibody [C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID20371
- Target nameFoxp3
- Target descriptionforkhead box P3
- Target synonymsJM2, scurfin, sf, forkhead box protein P3, scurfy
- HostRabbit
- IsotypeIgG
- Protein IDQ99JB6
- Protein NameForkhead box protein P3
- Scientific DescriptionProbable transcription factor. Plays a critical role in the control of immune response.
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Chikaishi W, Higashi T, Hayashi H, et al. Adiponectin-expressing Treg-containing T cell fraction inhibits tumor growth in orthotopically implanted triple-negative breast cancer. Thorac Cancer. 2023,14(30):3058-3062. doi: 10.1111/1759-7714.15102Read this paper
- Chikaishi W, Higashi T, Hayashi H, et al. Potential activity of adiponectin-expressing regulatory T cells against triple-negative breast cancer cells through the cell-in-cell phenomenon. Thorac Cancer. 2023,14(20):1941-1945. doi: 10.1111/1759-7714.14940Read this paper
- Hanamatsu Y, Saigo C, Takeuchi T. Adiponectin-expressing Treg cells in experimental thymic tumor model. Thorac Cancer. 2023,14(4):432-433. doi: 10.1111/1759-7714.14792Read this paper
- Yen YT, Chien M, Wu PY, et al. Protein phosphatase 2A inactivation induces microsatellite instability, neoantigen production and immune response. Nat Commun. 2021,12(1):7297. doi: 10.1038/s41467-021-27620-xRead this paper
- Rana MN, Lu J, Xue E, et al. PDE9 Inhibitor PF-04447943 Attenuates DSS-Induced Colitis by Suppressing Oxidative Stress, Inflammation, and Regulating T-Cell Polarization. Front Pharmacol. 2021,12:643215. doi: 10.3389/fphar.2021.643215Read this paper
- Ko M, Kamimura K, Owaki T, et al. Modulation of serotonin in the gut-liver neural axis ameliorates the fatty and fibrotic changes in non-alcoholic fatty liver. Dis Model Mech. 2021,14(3). doi: 10.1242/dmm.048922Read this paper
- Zhang L, Li Q, Xu J, et al. Cimetidine promotes STUB1-mediated degradation of tumoral FOXP3 by activating PI3K-Akt pathway in gastric cancer. Ann Transl Med. 2020,8(20):1304. doi: 10.21037/atm-20-6070Read this paper
- Jin X, Lin T, Yang G, et al. Use of Tregs as a cell-based therapy via CD39 for benign prostate hyperplasia with inflammation. J Cell Mol Med. 2020,24(9):5082-5096. doi: 10.1111/jcmm.15137Read this paper
- Nguyen DJM, Theodoropoulos G, Li YY, et al. Targeting the Kynurenine Pathway for the Treatment of Cisplatin-Resistant Lung Cancer. Mol Cancer Res. 2020,18(1):105-117. doi: 10.1158/1541-7786.MCR-19-0239Read this paper
![FOXP3 antibody [C3], C-term detects FOXP3 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FOXP3 protein stained by FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm. FOXP3 antibody [C3], C-term detects FOXP3 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FOXP3 protein stained by FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX107737/GTX107737_39715_20160303_IFA_w_23060120_986.webp)
![Various whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program. Various whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FOXP3 antibody [C3], C-term (GTX107737) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX107737/GTX107737_39715_20250502_WB_TPM_watermark_25050623_586.webp)



![FACS analysis of mouse C57Bl/6 splenocytes using GTX01488-07 FOXP3 antibody [MF23] (APC). Right panel : primary antibody Left panel : isotype control antibody amount : 0.25 μg (5 μl)](https://www.genetex.com/upload/website/prouct_img/normal/GTX01488-07/GTX01488-07_20200428_FACS9_w_23053121_490.webp)
![FACS analysis of mouse C57Bl/6 splenocytes using GTX01488-08 FOXP3 antibody [MF23] (PE). Right panel : primary antibody Left panel : isotype control antibody amount : 0.25 μg (5 μl)](https://www.genetex.com/upload/website/prouct_img/normal/GTX01488-08/GTX01488-08_20200428_FACS99_w_23053121_811.webp)
![FACS analysis of C57Bl/6 splenocytes stained FITC anti-mouse CD4 antibody, followed by intracellular staining with GTX80283-07 FOXP3 antibody [3G3] (APC). Right panel : Co-stained with FITC anti-mouse CD4 antibody and GTX80283-07 Left panel : Co-stained with FITC anti-mouse CD4 antibody and APC mouse IgG1 isotype control Antibody amount : 0.125 μg](https://www.genetex.com/upload/website/prouct_img/normal/GTX80283-07/GTX80283-07_20201020_FACS_11_w_23061322_251.webp)

![FACS analysis of C57Bl/6 splenocytes stained APC anti-mouse CD4 antibody, followed by intracellular staining with GTX80284 FOXP3 antibody [3G3] (PE). Right panel : Co-stained with APC anti-mouse CD4 antibody and GTX80284 Left panel : Co-stained with APC anti-mouse CD4 antibody and PE mouse IgG1 isotype control Antibody amount : 0.125 μg](https://www.genetex.com/upload/website/prouct_img/normal/GTX80284/GTX80284_20201020_FACS_64_w_23061322_985.webp)