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FLG / Filaggrin Antibody

LS-C783376
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetFLG
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FLG / Filaggrin Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (0.1 - 0.5 µg/ml) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2312
  • Target name
    FLG
  • Target description
    filaggrin
  • Target synonyms
    ATOD2; epidermal filaggrin; filaggrin
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Filaggrin using anti-Filaggrin antibody (PB9870). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: 22RV1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Filaggrin antigen affinity purified polyclonal antibody (Catalog # PB9870) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Filaggrin at approximately 435 kDa. The expected band size for Filaggrin is at 435 kDa.
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