Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody (GTX112937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![FLI1 antibody immunoprecipitates FLI1 protein in IP experiments. IP samples: Raji whole cell extract A. 40 μg Raji whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of FLI1 protein by 4 μg FLI1 antibody (GTX112937) 10 % SDS-PAGE The immunoprecipitated FLI1 protein was detected by FLI1 antibody (GTX112937) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX112937/GTX112937_40758_IP_w_23060500_866.webp "FLI1 antibody immunoprecipitates FLI1 protein in IP experiments. IP samples: Raji whole cell extract A. 40 μg Raji whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of FLI1 protein by 4 μg FLI1 antibody (GTX112937) 10 % SDS-PAGE The immunoprecipitated FLI1 protein was detected by FLI1 antibody (GTX112937) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]")
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FLI1 antibody (GTX112937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
FLI1 antibody
GTX112937
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetFLI1
Overview
- SupplierGeneTex
- Product NameFLI1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.22 mg/ml
- ConjugateUnconjugated
- Gene ID2313
- Target nameFLI1
- Target descriptionFli-1 proto-oncogene, ETS transcription factor
- Target synonymsBDPLT21, EWSR2, FLI-1, SIC-1, Friend leukemia integration 1 transcription factor, Ewing sarcoma breakpoint region 2, Friend leukemia virus integration 1, transcription factor ERGB
- HostRabbit
- IsotypeIgG
- Protein IDQ01543
- Protein NameFriend leukemia integration 1 transcription factor
- Scientific DescriptionSequence-specific transcriptional activator. Recognizes the DNA sequence 5-C[CA]GGAAGT-3.
- ReactivityHuman, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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![WB analysis of THP1 and Raji cell lysates using GTX34734 FLI1 antibody [FLI1/1312].](https://www.genetex.com/upload/website/prouct_img/normal/GTX34734/GTX34734_20200115_WB_1886_w_23060801_927.webp)
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