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FLI1 antibody

GTX112937
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetFLI1
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Overview

  • Supplier
    GeneTex
  • Product Name
    FLI1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.22 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2313
  • Target name
    FLI1
  • Target description
    Fli-1 proto-oncogene, ETS transcription factor
  • Target synonyms
    BDPLT21; Ewing sarcoma breakpoint region 2; EWSR2; Friend leukemia integration 1 transcription factor; Friend leukemia virus integration 1; SIC-1; transcription factor ERGB
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01543
  • Protein Name
    Friend leukemia integration 1 transcription factor
  • Scientific Description
    Sequence-specific transcriptional activator. Recognizes the DNA sequence 5-C[CA]GGAAGT-3.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FLI1 using anti-FLI1 antibody (A00399). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLI1 antigen affinity purified polyclonal antibody (Catalog # A00399) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLI1 at approximately 54KD. The expected band size for FLI1 is at 51KD.
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