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Western Blot analysis of 3T3 cells using Fli-1 Polyclonal Antibody
Western Blot analysis of 3T3 cells using Fli-1 Polyclonal Antibody
Western Blot analysis of 3T3 cells using Fli-1 Polyclonal Antibody

FLI1 Antibody

CSB-PA002539
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetFLI1
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Overview

  • Supplier
    Cusabio
  • Product Name
    FLI1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2313
  • Target name
    FLI1
  • Target description
    Fli-1 proto-oncogene, ETS transcription factor
  • Target synonyms
    BDPLT21; Ewing sarcoma breakpoint region 2; EWSR2; Friend leukemia integration 1 transcription factor; Friend leukemia virus integration 1; SIC-1; transcription factor ERGB
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01543
  • Protein Name
    Friend leukemia integration 1 transcription factor
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FLI1 using anti-FLI1 antibody (A00399). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLI1 antigen affinity purified polyclonal antibody (Catalog # A00399) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLI1 at approximately 54KD. The expected band size for FLI1 is at 51KD.
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