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Western Blot analysis of A549 cells using HNF-3alpha/beta/gamma Polyclonal Antibody
Western Blot analysis of A549 cells using HNF-3alpha/beta/gamma Polyclonal Antibody
Western Blot analysis of A549 cells using HNF-3alpha/beta/gamma Polyclonal Antibody

FOXA1 Antibody

CSB-PA005476
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFOXA1
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Overview

  • Supplier
    Cusabio
  • Product Name
    FOXA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3169
  • Target name
    FOXA1
  • Target description
    forkhead box A1
  • Target synonyms
    forkhead box protein A1; hepatocyte nuclear factor 3-alpha; HNF3A; HNF-3A; HNF-3-alpha; TCF3A; TCF-3A; transcription factor 3A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55317
  • Protein Name
    Hepatocyte nuclear factor 3-alpha
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FOXA1 using anti-FOXA1 antibody (A00266-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 cell lysate, Lane 2: human A549 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXA1 antigen affinity purified polyclonal antibody (Catalog # A00266-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXA1 at approximately 49KD. The expected band size for FOXA1 is at 49KD.
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