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Lane 1: 293 lysates; Lane 2: Mouse lung lysates probed with FOXA1 (5F7) Monoclonal Antibody (bsm-52072R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Lane 1: 293 lysates; Lane 2: Mouse lung lysates probed with FOXA1 (5F7) Monoclonal Antibody (bsm-52072R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Lane 1: 293 lysates; Lane 2: Mouse lung lysates probed with FOXA1 (5F7) Monoclonal Antibody (bsm-52072R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.

FOXA1 Recombinant Antibody

BSM-52072R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetFOXA1
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Overview

  • Supplier
    Bioss
  • Product Name
    FOXA1 Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(), ICC/IF(1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID3169
  • Target name
    FOXA1
  • Target description
    forkhead box A1
  • Target synonyms
    HNF3A, TCF3A, hepatocyte nuclear factor 3-alpha, HNF-3-alpha, HNF-3A, TCF-3A, forkhead box protein A1, transcription factor 3A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55317
  • Protein Name
    Hepatocyte nuclear factor 3-alpha
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of FOXA1 using anti-FOXA1 antibody (A00266-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 cell lysate, Lane 2: human A549 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXA1 antigen affinity purified polyclonal antibody (Catalog # A00266-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXA1 at approximately 49KD. The expected band size for FOXA1 is at 49KD.
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