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FACS analysis of PFA fixed MCF-7 cells using GTX02640 FOXA1 antibody [FOXA1/2230R]. Blue : Primary antibody Red : Isotype control
FACS analysis of PFA fixed MCF-7 cells using GTX02640 FOXA1 antibody [FOXA1/2230R]. Blue : Primary antibody Red : Isotype control
FACS analysis of PFA fixed MCF-7 cells using GTX02640 FOXA1 antibody [FOXA1/2230R]. Blue : Primary antibody Red : Isotype control

FOXA1 antibody [FOXA1/2230R]

GTX02640
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetFOXA1
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Overview

  • Supplier
    GeneTex
  • Product Name
    FOXA1 antibody [FOXA1/2230R]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1-2 microg/ml. ICC/IF: 1-2 microg/ml. IHC-P: 1-2 microg/ml. FCM: 0.5-1 microg/million cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    FOXA1/2230R
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID3169
  • Target name
    FOXA1
  • Target description
    forkhead box A1
  • Target synonyms
    HNF3A, TCF3A, hepatocyte nuclear factor 3-alpha, HNF-3-alpha, HNF-3A, TCF-3A, forkhead box protein A1, transcription factor 3A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55317
  • Protein Name
    Hepatocyte nuclear factor 3-alpha
  • Scientific Description
    This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FOXA1 using anti-FOXA1 antibody (A00266-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 cell lysate, Lane 2: human A549 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXA1 antigen affinity purified polyclonal antibody (Catalog # A00266-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXA1 at approximately 49KD. The expected band size for FOXA1 is at 49KD.
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