Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FOXA1 antibody [HL1974] (GTX637867) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
![FOXA1 antibody [HL1974] detects FOXA1 protein at nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FOXA1 stained by FOXA1 antibody [HL1974] (GTX637867) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637867/GTX637867_T-44865_20230414_ICC_IF_23041719_136.webp "FOXA1 antibody [HL1974] detects FOXA1 protein at nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FOXA1 stained by FOXA1 antibody [HL1974] (GTX637867) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.")
![Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FOXA1 antibody [HL1974] (GTX637867) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637867/GTX637867_44928_20231229_WB_shRNA_watermark_24010223_606.webp "Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FOXA1 antibody [HL1974] (GTX637867) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FOXA1 antibody [HL1974] (GTX637867) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
FOXA1 antibody [HL1974]
GTX637867
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetFOXA1
Overview
- SupplierGeneTex
- Product NameFOXA1 antibody [HL1974]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1974
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3169
- Target nameFOXA1
- Target descriptionforkhead box A1
- Target synonymsHNF3A, TCF3A, hepatocyte nuclear factor 3-alpha, HNF-3-alpha, HNF-3A, TCF-3A, forkhead box protein A1, transcription factor 3A
- HostRabbit
- IsotypeIgG
- Protein IDP55317
- Protein NameHepatocyte nuclear factor 3-alpha
- Scientific DescriptionThis gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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