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ProductsBack/FOXA1 Mouse Monoclonal Antibody [Clone ID: OTI3A8]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXA1 (RC206045, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXA1. Positive lysates LY401427 (100ug) and LC401427 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXA1 (RC206045, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXA1. Positive lysates LY401427 (100ug) and LC401427 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXA1 (RC206045, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXA1. Positive lysates LY401427 (100ug) and LC401427 (20ug) can be purchased separately from OriGene.

FOXA1 Mouse Monoclonal Antibody [Clone ID: OTI3A8]

CF500245
OriGene
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetFOXA1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    FOXA1 Mouse Monoclonal Antibody [Clone ID: OTI3A8]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3A8
  • Gene ID3169
  • Target name
    FOXA1
  • Target description
    forkhead box A1
  • Target synonyms
    HNF3A, TCF3A, hepatocyte nuclear factor 3-alpha, HNF-3-alpha, HNF-3A, TCF-3A, forkhead box protein A1, transcription factor 3A
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP55317
  • Protein Name
    Hepatocyte nuclear factor 3-alpha
  • Scientific Description
    Carrier-free (BSA/glycerol-free) FOXA1 mouse monoclonal antibody, clone OTI3A8 (formerly 3A8)
  • Reactivity
    Canine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of FOXA1 using anti-FOXA1 antibody (A00266-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 cell lysate, Lane 2: human A549 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXA1 antigen affinity purified polyclonal antibody (Catalog # A00266-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXA1 at approximately 49KD. The expected band size for FOXA1 is at 49KD.
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