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IHC-P analysis of human thyroid Gland using GTX25080 FOXE1 antibody, C-term. Antigen retrieval : Tris/EDTA buffer pH 9 Dilution : 2microg/ml
IHC-P analysis of human thyroid Gland using GTX25080 FOXE1 antibody, C-term. Antigen retrieval : Tris/EDTA buffer pH 9 Dilution : 2microg/ml
IHC-P analysis of human thyroid Gland using GTX25080 FOXE1 antibody, C-term. Antigen retrieval : Tris/EDTA buffer pH 9 Dilution : 2microg/ml

FOXE1 antibody, C-term

GTX25080
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetFOXE1
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Overview

  • Supplier
    GeneTex
  • Product Name
    FOXE1 antibody, C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 5microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2304
  • Target name
    FOXE1
  • Target description
    forkhead box E1
  • Target synonyms
    FKHL15; forkhead box protein E1; forkhead box protein E2; forkhead, drosophila, homolog-like 15; forkhead-related protein FKHL15; FOXE2; HFKH4; HFKL5; HNF-3/fork head-like protein 5; NMTC4; thyroid transcription factor 2; TITF2; TTF2; TTF-2
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDO00358
  • Protein Name
    Forkhead box protein E1
  • Scientific Description
    This intronless gene encodes a protein that belongs to the forkhead family of transcription factors. Members of this family contain a conserved 100-amino acid DNA-binding forkhead domain. The encoded protein functions as a thyroid transcription factor that plays a role in thyroid morphogenesis. Mutations in this gene are associated with the Bamforth-Lazarus syndrome, and with susceptibility to nonmedullary thyroid cancer-4. [provided by RefSeq, Nov 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FOXE1 using anti-FOXE1 antibody (A02831-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXE1 antigen affinity purified polyclonal antibody (Catalog # A02831-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXE1 at approximately 41KD. The expected band size for FOXE1 is at 38KD.
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