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FOXE1 antibody immunoprecipitates FOXE1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of FOXE1 protein by 2 μg of FOXE1 antibody (GTX100275) 15% SDS-PAGE The immunoprecipitated FOXE1 protein was detected by FOXE1 antibody (GTX100275) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
FOXE1 antibody immunoprecipitates FOXE1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of FOXE1 protein by 2 μg of FOXE1 antibody (GTX100275) 15% SDS-PAGE The immunoprecipitated FOXE1 protein was detected by FOXE1 antibody (GTX100275) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
FOXE1 antibody immunoprecipitates FOXE1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of FOXE1 protein by 2 μg of FOXE1 antibody (GTX100275) 15% SDS-PAGE The immunoprecipitated FOXE1 protein was detected by FOXE1 antibody (GTX100275) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

FOXE1 antibody

GTX100275
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetFOXE1
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Overview

  • Supplier
    GeneTex
  • Product Name
    FOXE1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2304
  • Target name
    FOXE1
  • Target description
    forkhead box E1
  • Target synonyms
    BAMLAZ, FKHL15, FOXE2, HFKH4, HFKL5, NMTC4, TITF2, TTF-2, TTF2, forkhead box protein E1, HNF-3/fork head-like protein 5, forkhead box protein E2, forkhead, drosophila, homolog-like 15, forkhead-related protein FKHL15, thyroid transcription factor 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00358
  • Protein Name
    Forkhead box protein E1
  • Scientific Description
    This intronless gene belongs to the forkhead family of transcription factors, which is characterized by a distinct forkhead domain. This gene functions as a thyroid transcription factor which likely plays a crucial role in thyroid morphogenesis. Mutations in this gene are associated with congenital hypothyroidism and cleft palate with thyroid dysgenesis. The map localization of this gene suggests it may also be a candidate gene for squamous cell epithelioma and hereditary sensory neuropathy type I. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FOXE1 using anti-FOXE1 antibody (A02831-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXE1 antigen affinity purified polyclonal antibody (Catalog # A02831-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXE1 at approximately 41KD. The expected band size for FOXE1 is at 38KD.
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