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ProductsBack/FRK Mouse Monoclonal Antibody [Clone ID: OTI3E2]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FRK (RC204460, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FRK. Positive lysates LY400740 (100ug) and LC400740 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FRK (RC204460, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FRK. Positive lysates LY400740 (100ug) and LC400740 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FRK (RC204460, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FRK. Positive lysates LY400740 (100ug) and LC400740 (20ug) can be purchased separately from OriGene.

FRK Mouse Monoclonal Antibody [Clone ID: OTI3E2]

CF501052
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFRK
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    FRK Mouse Monoclonal Antibody [Clone ID: OTI3E2]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3E2
  • Gene ID2444
  • Target name
    FRK
  • Target description
    fyn related Src family tyrosine kinase
  • Target synonyms
    GTK, PTK5, RAK, tyrosine-protein kinase FRK, PTK5 protein tyrosine kinase 5, fyn-related kinase, nuclear tyrosine protein kinase RAK, protein-tyrosine kinase 5
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP42685
  • Protein Name
    Tyrosine-protein kinase FRK
  • Scientific Description
    Carrier-free (BSA/glycerol-free) FRK mouse monoclonal antibody, clone OTI3E2 (formerly 3E2)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of FRK using anti-FRK antibody (A04075-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRK antigen affinity purified polyclonal antibody (Catalog # A04075-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRK at approximately 58 kDa. The expected band size for FRK is at 58 kDa.
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