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G9a antibody

GTX129153
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetEHMT2
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Overview

  • Supplier
    GeneTex
  • Product Name
    G9a antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.34 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10919
  • Target name
    EHMT2
  • Target description
    euchromatic histone lysine methyltransferase 2
  • Target synonyms
    BAT8, C6orf30, G9A, GAT8, KMT1C, NG36, histone-lysine N-methyltransferase EHMT2, G9A histone methyltransferase, H3-K9-HMTase 3, HLA-B associated transcript 8, ankyrin repeat-containing protein, euchromatic histone-lysine N-methyltransferase 2, histone H3-K9 methyltransferase 3, histone-lysine N-methyltransferase, H3 lysine-9 specific 3, lysine N-methyltransferase 1C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96KQ7
  • Protein Name
    Histone-lysine N-methyltransferase EHMT2
  • Scientific Description
    This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Yan F, Chen Z, Cui W. H3K9me2 regulation of BDNF expression via G9a partakes in the progression of heart failure. BMC Cardiovasc Disord. 2022,22(1):182. doi: 10.1186/s12872-022-02621-w
    Read this paper
  • Lin HY, Wu HJ, Chen SY, et al. Epigenetic therapy combination of UNC0638 and CI-994 suppresses breast cancer via epigenetic remodeling of BIRC5 and GADD45A. Biomed Pharmacother. 2022,145:112431. doi: 10.1016/j.biopha.2021.112431
    Read this paper
  • Zhang K, Wang J, Yang L, et al. Targeting histone methyltransferase G9a inhibits growth and Wnt signaling pathway by epigenetically regulating HP1α and APC2 gene expression in non-small cell lung cancer. Mol Cancer. 2018,17(1):153. doi: 10.1186/s12943-018-0896-8
    Read this paper
  • Tian YF, Wang HC, Luo CW, et al. Preprogramming therapeutic response of PI3K/mTOR dual inhibitor via the regulation of EHMT2 and p27 in pancreatic cancer. Am J Cancer Res. 2018,8(9):1812-1822.
    Read this paper
  • Luo CW, Wang JY, Hung WC, et al. G9a governs colon cancer stem cell phenotype and chemoradioresistance through PP2A-RPA axis-mediated DNA damage response. Radiother Oncol. 2017,124(3):395-402. doi: 10.1016/j.radonc.2017.03.002
    Read this paper

Datasheet

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Figure 1. Western blot analysis of EHMT2/G9A using anti-EHMT2/G9A antibody (A01055-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EHMT2/G9A antigen affinity purified polyclonal antibody (Catalog # A01055-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EHMT2/G9A at approximately 160-180 kDa. The expected band size for EHMT2/G9A is at 160-180 kDa.
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