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Western Blot analysis of K562 cells using Gab 2 Polyclonal Antibody
Western Blot analysis of K562 cells using Gab 2 Polyclonal Antibody
Western Blot analysis of K562 cells using Gab 2 Polyclonal Antibody

GAB2 Antibody

CSB-PA002610
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGAB2
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Overview

  • Supplier
    Cusabio
  • Product Name
    GAB2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9846
  • Target name
    GAB2
  • Target description
    GRB2 associated binding protein 2
  • Target synonyms
    Grb2-associated binder 2; GRB2-associated-binding protein 2; growth factor receptor bound protein 2-associated protein 2; pp100
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UQC2
  • Protein Name
    GRB2-associated-binding protein 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GAB2 using anti-GAB2 antibody (A02386-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAB2 antigen affinity purified polyclonal antibody (Catalog # A02386-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAB2 at approximately 90 kDa. The expected band size for GAB2 is at 74 kDa.
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