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Formalin-fixed and paraffin embedded mouse spleen labeled with Anti-Gab2 Polyclonal Antibody, Unconjugated (bs-2885R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse spleen labeled with Anti-Gab2 Polyclonal Antibody, Unconjugated (bs-2885R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse spleen labeled with Anti-Gab2 Polyclonal Antibody, Unconjugated (bs-2885R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Gab2 Polyclonal Antibody

BS-2885R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetGAB2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Gab2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID9846
  • Target name
    GAB2
  • Target description
    GRB2 associated binding protein 2
  • Target synonyms
    Grb2-associated binder 2; GRB2-associated-binding protein 2; growth factor receptor bound protein 2-associated protein 2; pp100
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UQC2
  • Protein Name
    GRB2-associated-binding protein 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Berberine suppresses mast cell-mediated allergic responses via regulating FcepsilonRI-mediated and MAPK signaling. Fu S et al., 2019 Jun, Int Immunopharmacol
    Read more

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Figure 1. Western blot analysis of GAB2 using anti-GAB2 antibody (A02386-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAB2 antigen affinity purified polyclonal antibody (Catalog # A02386-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAB2 at approximately 90 kDa. The expected band size for GAB2 is at 74 kDa.
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