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WB analysis of mouse brain lysate using GTX88118 GABA A Receptor gamma 2 antibody, Internal. Dilution : 2microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of mouse brain lysate using GTX88118 GABA A Receptor gamma 2 antibody, Internal. Dilution : 2microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of mouse brain lysate using GTX88118 GABA A Receptor gamma 2 antibody, Internal. Dilution : 2microg/ml Loading : 35microg protein in RIPA buffer

GABA A Receptor gamma 2 antibody, Internal

GTX88118
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGABRG2
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Overview

  • Supplier
    GeneTex
  • Product Name
    GABA A Receptor gamma 2 antibody, Internal
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1-3microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2566
  • Target name
    GABRG2
  • Target description
    gamma-aminobutyric acid type A receptor subunit gamma2
  • Target synonyms
    CAE2, DEE74, ECA2, EIEE74, FEB8, GEFSP3, gamma-aminobutyric acid receptor subunit gamma-2, GABA(A) receptor subunit gamma-2, GABA(A) receptor, gamma 2, GABAAR subunit gamma-2, gamma-aminobutyric acid (GABA) A receptor, gamma 2, gamma-aminobutyric acid type A receptor gamma2 subunit
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP18507
  • Protein Name
    Gamma-aminobutyric acid receptor subunit gamma-2
  • Scientific Description
    This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GABA A Receptor gamma 2/GABRG2 using anti-GABA A Receptor gamma 2/GABRG2 antibody (A02361-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABA A Receptor gamma 2/GABRG2 antigen affinity purified polyclonal antibody (Catalog # A02361-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABA A Receptor gamma 2/GABRG2 at approximately 50KD. The expected band size for GABA A Receptor gamma 2/GABRG2 is at 50KD.
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