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Western Blot analysis of COS7 cells using GABAB R1 Polyclonal Antibody
Western Blot analysis of COS7 cells using GABAB R1 Polyclonal Antibody
Western Blot analysis of COS7 cells using GABAB R1 Polyclonal Antibody

GABBR1 Antibody

CSB-PA002621
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGABBR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    GABBR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2550
  • Target name
    GABBR1
  • Target description
    gamma-aminobutyric acid type B receptor subunit 1
  • Target synonyms
    GABA-B receptor, R1 subunit; GABABR1; GABBR1-3; gamma-aminobutyric acid (GABA) B receptor, 1; gamma-aminobutyric acid type B receptor subunit 1; GB1; GPRC3A; seven transmembrane helix receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBS5
  • Protein Name
    Gamma-aminobutyric acid type B receptor subunit 1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GABBR1 using anti-GABBR1 antibody (A07297-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABBR1 antigen affinity purified polyclonal antibody (Catalog # A07297-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABBR1 at approximately 108 kDa. The expected band size for GABBR1 is at 108,130 kDa.
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