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Goat anti-GABBR1

EB09093
Everest Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGABBR1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-GABBR1 Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID2550
  • Target name
    GABBR1
  • Target description
    gamma-aminobutyric acid type B receptor subunit 1
  • Target synonyms
    GABA-B receptor, R1 subunit; GABABR1; GABBR1-3; gamma-aminobutyric acid (GABA) B receptor, 1; gamma-aminobutyric acid type B receptor subunit 1; GB1; GPRC3A; seven transmembrane helix receptor
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001461.1; NP_068703.1; NP_068704.2; NP_068705.2. Purification: Antigen affinity purified. Names and symbols: GABBR1; gamma-aminobutyric acid (GABA) B receptor, 1; FLJ92613; GABAB(1e); GABABR1; GABBR1-3; GPRC3A; dJ271M21.1.1; dJ271M21.1.2; hGB1a; GABA-B receptor; GABAB, subunit 1c; OTTHUMP00000029114; OTTHUMP00000029115; OTTHUMP00000029116; OTTHUMP00000109099; ga
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GABBR1 using anti-GABBR1 antibody (A07297-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABBR1 antigen affinity purified polyclonal antibody (Catalog # A07297-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABBR1 at approximately 108 kDa. The expected band size for GABBR1 is at 108,130 kDa.
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