GADD153 antibody [N1C3]
GTX112827
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDDIT3
Overview
- SupplierGeneTex
- Product NameGADD153 antibody [N1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.3 mg/ml
- ConjugateUnconjugated
- Gene ID1649
- Target nameDDIT3
- Target descriptionDNA damage inducible transcript 3
- Target synonymsAltDDIT3, C/EBPzeta, CEBPZ, CHOP, CHOP-10, CHOP10, GADD153, DNA damage-inducible transcript 3 protein, C/EBP zeta, CCAAT/enhancer-binding protein homologous protein, alternative DDIT3 protein, c/EBP-homologous protein 10, growth arrest and DNA damage-inducible protein GADD153
- HostRabbit
- IsotypeIgG
- Protein IDP35638
- Protein NameDNA damage-inducible transcript 3 protein
- Scientific DescriptionThis gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
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- Huang CY, Deng JS, Huang WC, et al. Attenuation of Lipopolysaccharide-Induced Acute Lung Injury by Hispolon in Mice, Through Regulating the TLR4/PI3K/Akt/mTOR and Keap1/Nrf2/HO-1 Pathways, and Suppressing Oxidative Stress-Mediated ER Stress-Induced Apoptosis and Autophagy. Nutrients. 2020,12(6). doi: 10.3390/nu12061742Read this paper
- Chen JF, Tsai YT, Lai YH, et al. Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells. J Pharm Biomed Anal. 2020,187:113142. doi: 10.1016/j.jpba.2020.113142Read this paper
- Rauti R, Qaisiya M, Tiribelli C, et al. Bilirubin disrupts calcium homeostasis in neonatal hippocampal neurons: a new pathway of neurotoxicity. Arch Toxicol. 2020,94(3):845-855. doi: 10.1007/s00204-020-02659-9Read this paper
- Zhu R, Li X, Xu J, et al. Defective endoplasmic reticulum export causes proinsulin misfolding in pancreatic β cells. Mol Cell Endocrinol. 2019,493:110470. doi: 10.1016/j.mce.2019.110470Read this paper
- Li JF, Li YS, Zhang YY, et al. Regulation of P300 and HDAC1 on endoplasmic reticulum stress in isoniazid-induced HL-7702 hepatocyte injury. J Cell Physiol. 2019,234(9):15299-15307. doi: 10.1002/jcp.28175Read this paper
- Lin S, Yang L, Shi H, et al. Endoplasmic reticulum-targeting photosensitizer Hypericin confers chemo-sensitization towards oxaliplatin through inducing pro-death autophagy. Int J Biochem Cell Biol. 2017,87:54-68. doi: 10.1016/j.biocel.2017.04.001Read this paper
- Chiang MC, Cheng YC, Nicol CJ, et al. Rosiglitazone activation of PPARγ-dependent signaling is neuroprotective in mutant huntingtin expressing cells. Exp Cell Res. 2015,338(2):183-93. doi: 10.1016/j.yexcr.2015.09.005Read this paper
- Karam R, Lou CH, Kroeger H, et al. The unfolded protein response is shaped by the NMD pathway. EMBO Rep. 2015,16(5):599-609. doi: 10.15252/embr.201439696Read this paper
- Lin S, Zhang L, Lei K, et al. Development of a multifunctional luciferase reporters system for assessing endoplasmic reticulum-targeting photosensitive compounds. Cell Stress Chaperones. 2014,19(6):927-37. doi: 10.1007/s12192-014-0517-4Read this paper



![WB analysis of 30 ug of whole cell lysates from undifferentiated 3T3-L1 fibroblasts (Fib) and 3T3-L1 fibroblasts differentiated into adipocytes (Day 2 and Day 9 post-differentiation) using GTX11419 GADD153 antibody [9C8]. 3T3-L1 fibroblasts were cultured in differentiation medium for 3 days to generate adipocytes. Differentiated adipocytes were maintained in maturation media containing either 5mM glucose (normal glucose) or 30mM glucose (high glucose). Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX11419/GTX11419_1466_WB_w_23060501_426.webp)
