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GADD45A / GADD45 Antibody

LS-C812977
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetGADD45A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GADD45A / GADD45 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1647
  • Target name
    GADD45A
  • Target description
    growth arrest and DNA damage inducible alpha
  • Target synonyms
    DDIT1; DDIT-1; DNA damage-inducible transcript 1 protein; DNA damage-inducible transcript-1; GADD45; growth arrest and DNA damage-inducible protein GADD45 alpha; growth arrest and DNA-damage-inducible 45 alpha
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GADD45A using anti-GADD45A antibody (PB9945). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45A antigen affinity purified polyclonal antibody (Catalog # PB9945) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD45A at approximately 19 kDa. The expected band size for GADD45A is at 18 kDa.
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