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ProductsBack/Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a) Polyclonal Antibody

Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a) Polyclonal Antibody

CAU21458
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetGADD45A
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Overview

  • Supplier
    Biomatik
  • Product Name
    Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1647
  • Target name
    GADD45A
  • Target description
    growth arrest and DNA damage inducible alpha
  • Target synonyms
    DDIT1; DDIT-1; DNA damage-inducible transcript 1 protein; DNA damage-inducible transcript-1; GADD45; growth arrest and DNA damage-inducible protein GADD45 alpha; growth arrest and DNA-damage-inducible 45 alpha
  • Host
    Rabbit
  • Protein IDP24522
  • Protein Name
    Growth arrest and DNA damage-inducible protein GADD45 alpha
  • Scientific Description
    The Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GADD45A using anti-GADD45A antibody (PB9945). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45A antigen affinity purified polyclonal antibody (Catalog # PB9945) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD45A at approximately 19 kDa. The expected band size for GADD45A is at 18 kDa.
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