IHC-P analysis of human colon tissue using GTX22785 Galectin 3 antibody [A3A12]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:100
![IHC-P analysis of human ovarian carcinoma tissue using GTX22785 Galectin 3 antibody [A3A12]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX22785/GTX22785_1108_IHC-P_w_23060620_715.webp "IHC-P analysis of human ovarian carcinoma tissue using GTX22785 Galectin 3 antibody [A3A12]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:100")
![IHC-P analysis of mouse colon tissue using GTX22785 Galectin 3 antibody [A3A12]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX22785/GTX22785_1109_IHC-P_w_23060620_285.webp "IHC-P analysis of mouse colon tissue using GTX22785 Galectin 3 antibody [A3A12]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:20")
![WB analysis of 25 ug of MCF-7 (Lane 1), Hela (Lane 2), and NIH-3T3 cell lysates (Lane 3) using GTX22785 Galectin 3 antibody [A3A12]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22785/GTX22785_1591_WB_w_23060620_665.webp "WB analysis of 25 ug of MCF-7 (Lane 1), Hela (Lane 2), and NIH-3T3 cell lysates (Lane 3) using GTX22785 Galectin 3 antibody [A3A12]. Dilution : 1:1000")
![ICC/IF analysis of MCF-7 cells using GTX22785 Galectin 3 antibody [A3A12]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22785/GTX22785_436_ICC-IF_w_23060620_833.webp "ICC/IF analysis of MCF-7 cells using GTX22785 Galectin 3 antibody [A3A12]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 oC")
![ICC/IF analysis of NIH-3T3 cells using GTX22785 Galectin 3 antibody [A3A12]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22785/GTX22785_437_ICC-IF_w_23060620_225.webp "ICC/IF analysis of NIH-3T3 cells using GTX22785 Galectin 3 antibody [A3A12]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 oC")
![ICC/IF analysis of HeLa cells using GTX22785 Galectin 3 antibody [A3A12]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22785/GTX22785_438_ICC-IF_w_23060620_588.webp "ICC/IF analysis of HeLa cells using GTX22785 Galectin 3 antibody [A3A12]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 oC")
![IHC-Fr analysis of rat olfactory bulb tissue using GTX22785 Galectin 3 antibody [A3A12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX22785/GTX22785_960_IHC-Fr_w_23060620_148.webp "IHC-Fr analysis of rat olfactory bulb tissue using GTX22785 Galectin 3 antibody [A3A12].")
IHC-P analysis of human colon tissue using GTX22785 Galectin 3 antibody [A3A12]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:100
Galectin 3 antibody [A3A12]
GTX22785
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetLGALS3
Overview
- SupplierGeneTex
- Product NameGalectin 3 antibody [A3A12]
- Delivery Days Customer9
- Antibody SpecificityThe epitope for this antibody has been mapped to the first 58 amino acids of galectin-3.
- Application Supplier NoteWB: 1 microg/ml. ICC/IF: 1:20-1:200. IHC-P: 1:20-1:200. IHC-Fr: 6 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDA3A12
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3958
- Target nameLGALS3
- Target descriptiongalectin 3
- Target synonyms35 kDa lectin; advanced glycation end-product receptor 3; carbohydrate-binding protein 35; CBP35; epididymis secretory sperm binding protein; GAL3; galactose-specific lectin 3; GALBP; galectin-3; GALIG; IgE-binding protein; L31; laminin-binding protein; lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; MAC2; MAC-2 antigen
- HostMouse
- IsotypeIgG1
- Protein IDP17931
- Protein NameGalectin-3
- Scientific DescriptionThis gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203