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FACS analysis of Raji cells using GTX04779 GAP43 antibody. Blue : Primary antibody Green : Isotype control Red : Unlabelled sample Dilution : 1 microg/10? cells
FACS analysis of Raji cells using GTX04779 GAP43 antibody. Blue : Primary antibody Green : Isotype control Red : Unlabelled sample Dilution : 1 microg/10? cells
FACS analysis of Raji cells using GTX04779 GAP43 antibody. Blue : Primary antibody Green : Isotype control Red : Unlabelled sample Dilution : 1 microg/10? cells

GAP43 antibody

GTX04779
GeneTex
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetGap43
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Overview

  • Supplier
    GeneTex
  • Product Name
    GAP43 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.25 microg/mL. IHC-P: 0.5-1 microg/mL. FACS: 1-3 microg/106 cells. ELISA: 0.1-0.5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID14432
  • Target name
    Gap43
  • Target description
    growth associated protein 43
  • Target synonyms
    axonal membrane protein GAP-43; B-50; Ba; Basp2; brain abundant, membrane attached signal protein 2; calmodulin-binding protein P-57; GAP-; GAP-43; growth accentuating protein 43; neuromodulin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06837
  • Protein Name
    Neuromodulin
  • Scientific Description
    Predicted to enable calmodulin binding activity and phospholipid binding activity. Acts upstream of or within axon choice point recognition; cell fate commitment; and glial cell differentiation. Located in several cellular components, including axon; plasma membrane; and postsynaptic density. Is expressed in several structures, including alimentary system; future brain; genitourinary system; nervous system; and sensory organ. Orthologous to human GAP43 (growth associated protein 43). [provided by Alliance of Genome Resources, Apr 2022]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GAP43 using anti-GAP43 antibody (A01868-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse C6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAP43 antigen affinity purified polyclonal antibody (Catalog # A01868-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAP43 at approximately 45KD. The expected band size for GAP43 is at 24KD.
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