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Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-GAP-43 Polyclonal Antibody, Unconjugated (bs-0154R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-GAP-43 Polyclonal Antibody, Unconjugated (bs-0154R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-GAP-43 Polyclonal Antibody, Unconjugated (bs-0154R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

GAP43 Polyclonal Antibody

BS-0154R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetGap43
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    GAP43 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID14432
  • Target name
    Gap43
  • Target description
    growth associated protein 43
  • Target synonyms
    axonal membrane protein GAP-43; B-50; Ba; Basp2; brain abundant, membrane attached signal protein 2; calmodulin-binding protein P-57; GAP-; GAP-43; growth accentuating protein 43; neuromodulin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06837
  • Protein Name
    Neuromodulin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Effect of Frankincense Extract on Nerve Recovery in the Rat Sciatic Nerve Damage Model. Jiang X et al., 2016, Evid Based Complement Alternat Med
    Read more

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Figure 1. Western blot analysis of GAP43 using anti-GAP43 antibody (A01868-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse C6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAP43 antigen affinity purified polyclonal antibody (Catalog # A01868-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAP43 at approximately 45KD. The expected band size for GAP43 is at 24KD.
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