![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637966/GTX637966_T-44886_20221209_WB_M_R_23010400_421.webp)
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GAPDH antibody [HL2062]
GTX637966
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityBacteria, Canine, Feline, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Zebra Fish
TargetGAPDH
Overview
- SupplierGeneTex
- Product NameGAPDH antibody [HL2062]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:50000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2062
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2597
- Target nameGAPDH
- Target descriptionglyceraldehyde-3-phosphate dehydrogenase
- Target synonymsG3PD, GAPD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, OCAS, p38 component, Oct1 coactivator in S phase, 38 Kd component, aging-associated gene 9 protein, epididymis secretory sperm binding protein Li 162eP, peptidyl-cysteine S-nitrosylase GAPDH
- HostRabbit
- IsotypeIgG
- Protein IDP04406
- Protein NameGlyceraldehyde-3-phosphate dehydrogenase
- Scientific DescriptionThis gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
- ReactivityBacteria, Canine, Feline, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637966/GTX637966_T-44886_20221209_WB_Z_23010400_802.webp)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637966/GTX637966_T-44886_20221209_WB_D_C_23010400_984.webp)
![GAPDH antibody [HL2062] detects GAPDH protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GAPDH stained by GAPDH antibody [HL2062] (GTX637966) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). GAPDH antibody [HL2062] detects GAPDH protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GAPDH stained by GAPDH antibody [HL2062] (GTX637966) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637966/GTX637966_T-44886_20230317_ICC_IF_23041023_926.webp)
![E. Coli (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. E. Coli (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637966/GTX637966_44977_20231110_WB_E.coli_23111422_532.webp)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637966/GTX637966_44977_20231110_WB_multiple_species_23111422_664.webp)
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637966/GTX637966_45236_20240126_WB_Hamster_24090423_602.webp)
![Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637966/GTX637966_45236_20231124_WB_Sensitivity_24090423_898.webp)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637966/GTX637966_45236_20241004_WB_24100900_830.webp)
![Whole Japanese medaka extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Whole Japanese medaka extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637966/GTX637966_45236_20250815_WB_medaka_25082121_336.webp)

![ICC/IF analysis of NIH-3T3 cells using GTX03407 GAPDH antibody [GT1355]. Red : Primary antibody Blue : DAPI for nuclear staining Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX03407/GTX03407_ICCIF_1_w_23053123_726.webp)
![GAPDH antibody [GT239] (HRP) detects GAPDH antibody protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. GAPDH antibody [GT239] (HRP) (GTX627408-01) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX627408-01/GTX627408-01_41268_20170817_IHC-P_R_w_23061202_629.webp)

![WB analysis of HeLa (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate using GTX83245 GAPDH antibody [1A10].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83245/GTX83245_20170912_WB_w_23061322_915.webp)
