
1:2000 (0.5μg/mL) Ab dilution used in WB of 5μg/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6)
GAPDH antibody [GA1R]
GTX82560
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityBacteria, Chicken, Hamster, Human, Mouse, Rabbit, Rat, Yeast
TargetGAPDH
Overview
- SupplierGeneTex
- Product NameGAPDH antibody [GA1R]
- Delivery Days Customer9
- Application Supplier NoteRecommended Starting Dilutions:WB (with ECL): Use at a dilution of 1:1000-10000 Immunostaining: Use at a dilution of 1:500 - 1:2000For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGA1R
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2597
- Target nameGAPDH
- Target descriptionglyceraldehyde-3-phosphate dehydrogenase
- Target synonymsG3PD, GAPD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, OCAS, p38 component, Oct1 coactivator in S phase, 38 Kd component, aging-associated gene 9 protein, epididymis secretory sperm binding protein Li 162eP, peptidyl-cysteine S-nitrosylase GAPDH
- HostMouse
- IsotypeIgG1
- Protein IDP04406
- Protein NameGlyceraldehyde-3-phosphate dehydrogenase
- Scientific DescriptionThis gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
- ReactivityBacteria, Chicken, Hamster, Human, Mouse, Rabbit, Rat, Yeast
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Rowe AA, Chen X, Nettesheim ER, et al. Long-term progression of retinal degeneration in a preclinical model of CLN7 Batten disease as a baseline for testing clinical therapeutics. EBioMedicine. 2022,85:104314. doi: 10.1016/j.ebiom.2022.104314Read this paper


![ICC/IF analysis of NIH-3T3 cells using GTX03407 GAPDH antibody [GT1355]. Red : Primary antibody Blue : DAPI for nuclear staining Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX03407/GTX03407_ICCIF_1_w_23053123_726.webp)
![GAPDH antibody [GT239] (HRP) detects GAPDH antibody protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. GAPDH antibody [GT239] (HRP) (GTX627408-01) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX627408-01/GTX627408-01_41268_20170817_IHC-P_R_w_23061202_629.webp)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637966/GTX637966_T-44886_20221209_WB_M_R_23010400_421.webp)
![WB analysis of HeLa (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate using GTX83245 GAPDH antibody [1A10].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83245/GTX83245_20170912_WB_w_23061322_915.webp)
