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GATA1 Antibody

LS-C812615
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGATA1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GATA1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:10000), IF (1:200 - 1:1000), IHC (1:200 - 1:1000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2623
  • Target name
    GATA1
  • Target description
    GATA binding protein 1
  • Target synonyms
    CNSHA9, ERYF1, GATA-1, GF-1, GF1, HAEADA, NF-E1, NFE1, XLANP, XLTDA, XLTT, erythroid transcription factor, GATA-binding factor 1, NF-E1 DNA-binding protein, erythroid transcription factor 1, globin transcription factor 1, nuclear factor, erythroid 1, transcription factor GATA1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GATA1 using anti-GATA1 antibody (A00408-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA1 antigen affinity purified polyclonal antibody (Catalog # A00408-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA1 at approximately 45KD. The expected band size for GATA1 is at 45KD.
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