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WB analysis of rat fetal heart (1) and adult heart (2) tissues lysate using GTX83368 GATA4 antibody [6H10].
WB analysis of rat fetal heart (1) and adult heart (2) tissues lysate using GTX83368 GATA4 antibody [6H10].
WB analysis of rat fetal heart (1) and adult heart (2) tissues lysate using GTX83368 GATA4 antibody [6H10].

GATA4 antibody [6H10]

GTX83368
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetGATA4
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Overview

  • Supplier
    GeneTex
  • Product Name
    GATA4 antibody [6H10]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6H10
  • Conjugate
    Unconjugated
  • Gene ID2626
  • Target name
    GATA4
  • Target description
    GATA binding protein 4
  • Target synonyms
    ASD2; GATA-binding factor 4; TACHD; TOF; transcription factor GATA-4; VSD1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP43694
  • Protein Name
    Transcription factor GATA-4
  • Scientific Description
    This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GATA4 using anti-GATA4 antibody (A00499-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA4 antigen affinity purified polyclonal antibody (Catalog # A00499-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA4 at approximately 45KD. The expected band size for GATA4 is at 45KD.
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