
FACS analysis of NIH/3T3 cells using GTX54530 GATA4 antibody compared to an isotype control (blue). Dilution : 2 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
GATA4 antibody
GTX54530
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetGATA4
Overview
- SupplierGeneTex
- Product NameGATA4 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. ICC/IF: 1:50-1:200. IHC-P: 1:100-1:1000. FCM: 3-5 microg/106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2626
- Target nameGATA4
- Target descriptionGATA binding protein 4
- Target synonymsASD2, TACHD, TOF, VSD1, transcription factor GATA-4, GATA-binding factor 4
- HostRabbit
- IsotypeIgG
- Protein IDP43694
- Protein NameTranscription factor GATA-4
- Scientific DescriptionThis gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
















![WB analysis of rat fetal heart (1) and adult heart (2) tissues lysate using GTX83368 GATA4 antibody [6H10].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83368/GTX83368_20170912_WB_w_23061322_773.webp)
![WB analysis of various cell lines using GTX84460 GATA4 antibody [9F9]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84460/GTX84460_4381_WB_w_23061420_895.webp)