![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody [HL3470] (GTX641355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody [HL3470] (GTX641355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641355/GTX641355_T-45600_20241122_WB_R_24112622_975.webp)
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody [HL3470] (GTX641355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GEF-H1 antibody [HL3470]
GTX641355
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetARHGEF2
Overview
- SupplierGeneTex
- Product NameGEF-H1 antibody [HL3470]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3470
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID9181
- Target nameARHGEF2
- Target descriptionRho/Rac guanine nucleotide exchange factor 2
- Target synonymsGEF, GEF-H1, GEFH1, LFP40, Lfc, NEDMHM, P40, rho guanine nucleotide exchange factor 2, Rho/Rac guanine nucleotide exchange factor (GEF) 2, guanine nucleotide exchange factor H1, microtubule-regulated Rho-GEF, proliferating cell nucleolar antigen p40
- HostRabbit
- IsotypeIgG
- Protein IDQ92974
- Protein NameRho guanine nucleotide exchange factor 2
- Scientific DescriptionRho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody [HL3470] (GTX641355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody [HL3470] (GTX641355) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641355/GTX641355_T-45600_20241122_WB_M_24112622_197.webp)
![GEF-H1 antibody [HL3470] detects GEF-H1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GEF-H1 stained by GEF-H1 antibody [HL3470] (GTX641355) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). GEF-H1 antibody [HL3470] detects GEF-H1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GEF-H1 stained by GEF-H1 antibody [HL3470] (GTX641355) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX641355/GTX641355_T-45600_20241213_ICC_IF_24123021_509.webp)
![GEF-H1 antibody [HL3470] detects GEF-H1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. GEF-H1 stained by GEF-H1 antibody [HL3470] (GTX641355) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Corresponding RNA levels (RPKM) in the tissues are based on NCBI database. GEF-H1 antibody [HL3470] detects GEF-H1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. GEF-H1 stained by GEF-H1 antibody [HL3470] (GTX641355) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641355/GTX641355_T-45600_20250113_IHC-P_M_Multiple_RPKM_2_25020422_739.webp)
![GEF-H1 antibody [HL3470] detects GEF-H1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. GEF-H1 stained by GEF-H1 antibody [HL3470] (GTX641355) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Corresponding RNA levels (RPKM) in the tissues are based on NCBI database. GEF-H1 antibody [HL3470] detects GEF-H1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. GEF-H1 stained by GEF-H1 antibody [HL3470] (GTX641355) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641355/GTX641355_T-45600_20250113_IHC-P_M_Multiple_RPKM_1_25020422_341.webp)
![GEF-H1 antibody [HL3470] detects GEF-H1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat hippocampus. GEF-H1 stained by GEF-H1 antibody [HL3470] (GTX641355) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min GEF-H1 antibody [HL3470] detects GEF-H1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat hippocampus. GEF-H1 stained by GEF-H1 antibody [HL3470] (GTX641355) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX641355/GTX641355_T-45600_20250214_IHC-P_R_25031220_777.webp)


![GEF-H1 antibody [GT336] detects GEF-H1 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract 7.5 % SDS-PAGE GEF-H1 antibody [GT336] (GTX629398) dilution: 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX629398/GTX629398_41323_WB_w_23061202_354.webp)
![GEF-H1 antibody [GT1132] detects GEF-H1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GEF-H1 stained by GEF-H1 antibody [GT1132] (GTX629399) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX629399/GTX629399_41421_20180525_IHC-P_R_w_23061202_421.webp)



