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Ghr Polyclonal Antibody

CAC11136
Biomatik
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGHR
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Overview

  • Supplier
    Biomatik
  • Product Name
    GHR Antibody
  • Delivery Days Customer
    12
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC; Recommended dilution: IHC:1:20-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2690
  • Target name
    GHR
  • Target description
    growth hormone receptor
  • Target synonyms
    GH receptor; GHBP; GHIP; growth hormone binding protein; growth hormone receptor; mutant growth hormone receptor; serum binding protein; somatotropin receptor; truncated growth hormone receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10912
  • Protein Name
    Growth hormone receptor
  • Scientific Description
    The Ghr Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, IHC.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GHR using anti-GHR antibody (A00698). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GHR antigen affinity purified polyclonal antibody (Catalog # A00698) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GHR at approximately 80KD. The expected band size for GHR is at 72KD.
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