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Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-GHR Polyclonal Antibody, Unconjugated (bs-0654R) at 1:400, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-GHR Polyclonal Antibody, Unconjugated (bs-0654R) at 1:400, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-GHR Polyclonal Antibody, Unconjugated (bs-0654R) at 1:400, followed by conjugation to the secondary antibody and DAB staining

Growth hormone receptor Polyclonal Antibody

BS-0654R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGHR
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    GHR Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2690
  • Target name
    GHR
  • Target description
    growth hormone receptor
  • Target synonyms
    GH receptor; GHBP; GHIP; growth hormone binding protein; growth hormone receptor; mutant growth hormone receptor; serum binding protein; somatotropin receptor; truncated growth hormone receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10912
  • Protein Name
    Growth hormone receptor
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Comparative study of stress response, growth and development of uteri in post-weaning gilts challenged with zearalenone and estradiol benzoate. Zhou M et al., 2019 Nov, J Anim Physiol Anim Nutr (Berl)
    Read more
  • Seasonal expressions of growth hormone receptor, insulin-like growth factor 1 and insulin-like growth factor 1 receptor in the scented glands of the muskrats (Ondatra zibethicus). Xie W et al., 2019 Sep 15, Gen Comp Endocrinol
    Read more
  • Effects of zearalenone on the localization and expression of the growth hormone receptor gene in the uteri of post-weaning piglets. Zhou M et al., 2018 Jan, Asian-Australas J Anim Sci
    Read more
  • MicroRNA-126 participates in lipid metabolism in mammary epithelial cells. Chu M et al., 2017 Oct 15, Mol Cell Endocrinol
    Read more
  • Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production. Zhao Y et al., 2017 Feb, Theriogenology
    Read more
  • Generation of a miniature pig disease model for human Laron syndrome. Cui D et al., 2015 Oct 29, Sci Rep
    Read more
  • Effect of sericin on diabetic hippocampal growth hormone/insulin-like growth factor 1 axis. Chen Z et al., 2013 Jul 5, Neural Regen Res
    Read more

Datasheet

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Figure 1. Western blot analysis of GHR using anti-GHR antibody (A00698). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GHR antigen affinity purified polyclonal antibody (Catalog # A00698) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GHR at approximately 80KD. The expected band size for GHR is at 72KD.
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