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Western Blot analysis of SKOV3 cells using GHR Polyclonal Antibody.
Western Blot analysis of SKOV3 cells using GHR Polyclonal Antibody.
Western Blot analysis of SKOV3 cells using GHR Polyclonal Antibody.

GHR Antibody

CSB-PA006152
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGHR
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Overview

  • Supplier
    Cusabio
  • Product Name
    GHR Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2690
  • Target name
    GHR
  • Target description
    growth hormone receptor
  • Target synonyms
    GH receptor; GHBP; GHIP; growth hormone binding protein; growth hormone receptor; mutant growth hormone receptor; serum binding protein; somatotropin receptor; truncated growth hormone receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10912
  • Protein Name
    Growth hormone receptor
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GHR using anti-GHR antibody (A00698). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GHR antigen affinity purified polyclonal antibody (Catalog # A00698) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GHR at approximately 80KD. The expected band size for GHR is at 72KD.
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