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ProductsBack/GHR Recombinant Monoclonal Antibody
IHC image of CSB-RA978909A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA978909A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA978909A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

GHR Recombinant Monoclonal Antibody

CSB-RA978909A0HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGHR
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Overview

  • Supplier
    Cusabio
  • Product Name
    GHR Recombinant Monoclonal Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    10C11
  • Conjugate
    Unconjugated
  • Gene ID2690
  • Target name
    GHR
  • Target description
    growth hormone receptor
  • Target synonyms
    GH receptor; GHBP; GHIP; growth hormone binding protein; growth hormone receptor; mutant growth hormone receptor; serum binding protein; somatotropin receptor; truncated growth hormone receptor
  • Isotype
    IgG
  • Protein IDP10912
  • Protein Name
    Growth hormone receptor
  • Scientific Description
    Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway (By similarity).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GHR using anti-GHR antibody (A00698). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GHR antigen affinity purified polyclonal antibody (Catalog # A00698) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GHR at approximately 80KD. The expected band size for GHR is at 72KD.
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