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WB analysis of human HeLa cell lysate using GTX25951 GIPC1 antibody, C-term. Dilution : 0.3microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human HeLa cell lysate using GTX25951 GIPC1 antibody, C-term. Dilution : 0.3microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human HeLa cell lysate using GTX25951 GIPC1 antibody, C-term. Dilution : 0.3microg/ml Loading : 35microg protein in RIPA buffer

GIPC1 antibody, C-term

GTX25951
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetGIPC1
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Overview

  • Supplier
    GeneTex
  • Product Name
    GIPC1 antibody, C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.03-0.1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10755
  • Target name
    GIPC1
  • Target description
    GIPC PDZ domain containing family member 1
  • Target synonyms
    C19orf3, GIPC, GLUT1CBP, Hs.6454, IIP-1, NIP, OPDM2, RGS19IP1, SEMCAP, SYNECTIIN, SYNECTIN, TIP-2, PDZ domain-containing protein GIPC1, GAIP C-terminus-interacting protein, GLUT1 C-terminal binding protein, IGF-1 receptor interacting protein 1, RGS-GAIP-interacting protein, RGS19-interacting protein 1, regulator of G-protein signalling 19 interacting protein 1, tax interaction protein 2
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDO14908
  • Protein Name
    PDZ domain-containing protein GIPC1
  • Scientific Description
    GIPC1 is a scaffolding protein that regulates cell surface receptor expression and trafficking (Lee et al., 2008 [PubMed 18775991]).[supplied by OMIM, Apr 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GIPC1 using anti-GIPC1 antibody (A04969-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat skeletal muscle tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: rat NRK whole cell lysates, Lane 6: mouse skeletal muscle tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIPC1 antigen affinity purified polyclonal antibody (Catalog # A04969-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GIPC1 at approximately 40 kDa. The expected band size for GIPC1 is at 36 kDa.
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