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Western blot analysis of extracts of various cell lines using GLDC Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using GLDC Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using GLDC Polyclonal Antibody at dilution of 1:1000.

GLDC Polyclonal Antibody

E-AB-62761
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    GLDC Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Glycine decarboxylase/GLDC using anti-Glycine decarboxylase/GLDC antibody (A04777-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEPG2 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human T47D whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse liver tissue lysates, Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glycine decarboxylase/GLDC antigen affinity purified polyclonal antibody (Catalog # A04777-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glycine decarboxylase/GLDC at approximately 113KD. The expected band size for Glycine decarboxylase/GLDC is at 113KD.
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ReactivityHuman, Mouse, Rat
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TargetGLDC
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TargetGLDC
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Immunohistochemistry analysis in human placenta and skeletal muscle tissues using HPA002318 antibody. Corresponding GLDC RNA-seq data are presented for the same tissues.
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IHC image of CSB-PA328400LA01HU diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunoprecipitation of Glycine dehydrogenase protein from HepG2 whole cell extracts using 5 μg of Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267). Western blot analysis was performed using Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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