More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/GLI / GLI1 Antibody

GLI / GLI1 Antibody

LS-C813273
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetGLI1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GLI / GLI1 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:5000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2735
  • Target name
    GLI1
  • Target description
    GLI family zinc finger 1
  • Target synonyms
    GLI; GLI-Kruppel family member GLI1; glioma-associated oncogene 1; glioma-associated oncogene homolog 1 (zinc finger protein); oncogene GLI; PAPA8; PPD1; zinc finger protein GLI1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
RayBiotech
Anti-GLI1 Antibody144-08387
ApplicationsWestern Blot
TargetGLI1
  • SizePrice
Anti-Gli1 Antibody
Product group Antibodies
Antibodies.com
Anti-Gli1 AntibodyA121161
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
  • SizePrice
Figure 1. Western blot analysis of GLI1 using anti-GLI1 antibody (A00527-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLI1 antigen affinity purified polyclonal antibody (Catalog # A00527-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLI1 at approximately 150 kDa. The expected band size for GLI1 is at 118 kDa.
Product group Antibodies
Boster Bio
Anti-GLI1 Antibody Picoband(r)A00527-3-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetGLI1
  • SizePrice
Product group Antibodies
Assay Genie
GLI1 AntibodyCAB14675
TargetGLI1
  • SizePrice
IHC image of CSB-PA12989A0Rb diluted at 1:400 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
GLI1 AntibodyCSB-PA12989A0RB
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetGLI1
  • SizePrice
Immunofluorescence staining of RH-30 cells using the Anti-GLI1 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Product group Antibodies
Atlas Antibodies
Anti-GLI1 AntibodyAMAB91771
ApplicationsWestern Blot, ImmunoCytoChemistry
ReactivityHuman
TargetGLI1
  • SizePrice
Product group Antibodies
Nordic-MUbio
Rabbit anti Human Zinc Finger Protein GLI1X2363P
ApplicationsWestern Blot
TargetGLI1
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-GLI1EB10594
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
TargetGLI1
  • SizePrice
Product group Antibodies
References
GeneTex
Gli1 antibodyGTX106207
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetGLI1
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GLI1 Polyclonal Antibody, Unconjugated (bs-1206R) at 1:400 overnight at 4°C, DAB staining.
Product group Antibodies
References
Bioss Antibodies
GLI1 Polyclonal AntibodyBS-1206R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetGLI1
  • SizePrice