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Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GLI1 Polyclonal Antibody, Unconjugated (bs-1206R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GLI1 Polyclonal Antibody, Unconjugated (bs-1206R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GLI1 Polyclonal Antibody, Unconjugated (bs-1206R) at 1:400 overnight at 4°C, DAB staining.

GLI1 Polyclonal Antibody

BS-1206R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Rabbit, Rat
TargetGLI1
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Overview

  • Supplier
    Bioss
  • Product Name
    GLI1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2735
  • Target name
    GLI1
  • Target description
    GLI family zinc finger 1
  • Target synonyms
    GLI, PAPA8, PPD1, zinc finger protein GLI1, GLI-Kruppel family member GLI1, glioma-associated oncogene 1, glioma-associated oncogene homolog 1 (zinc finger protein), oncogene GLI
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08151
  • Protein Name
    Zinc finger protein GLI1
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • Baicalin improves the in vitro developmental capacity of pig embryos by inhibiting apoptosis, regulating mitochondrial activity and activating sonic hedgehog signaling. Guo Q et al., 2019 Sep 1, Mol Hum Reprod
    Read this paper
  • Hesperetin derivative attenuates CCl4-induced hepatic fibrosis and inflammation by Gli-1-dependent mechanisms. Chen X et al., 2019 Nov, Int Immunopharmacol
    Read this paper
  • Blockade of Hedgehog Signaling Attenuates Biliary Cystogenesis in the Polycystic Kidney (PCK) Rat. Sato Y et al., 2018 Oct, Am J Pathol
    Read this paper
  • Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats. Yang H et al., 2017 Nov 21, Stem Cell Res Ther
    Read this paper
  • Doxorubicin combined with low intensity ultrasound suppresses the growth of oral squamous cell carcinoma in culture and in xenografts. Fan H et al., 2017 Nov 21, J Exp Clin Cancer Res
    Read this paper
  • LKB1 Regulates Cerebellar Development by Controlling Sonic Hedgehog-mediated Granule Cell Precursor Proliferation and Granule Cell Migration. Men Y et al., 2015 Nov 9, Sci Rep
    Read this paper
  • Suppressor of fused (Sufu) represses Gli1 transcription and nuclear accumulation, inhibits glioma cell proliferation, invasion and vasculogenic mimicry, improving glioma chemo-sensitivity and prognosis. Liu X et al., 2014 Nov 30, Oncotarget
    Read this paper
  • Targeting the SMO oncogene by miR-326 inhibits glioma biological behaviors and stemness. Du W et al., 2015 Feb, Neuro Oncol
    Read this paper

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