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Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GLI1 Polyclonal Antibody, Unconjugated (bs-1206R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GLI1 Polyclonal Antibody, Unconjugated (bs-1206R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GLI1 Polyclonal Antibody, Unconjugated (bs-1206R) at 1:400 overnight at 4°C, DAB staining.

GLI1 Polyclonal Antibody

BS-1206R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGLI1
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Overview

  • Supplier
    Bioss
  • Product Name
    GLI1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2735
  • Target name
    GLI1
  • Target description
    GLI family zinc finger 1
  • Target synonyms
    GLI, PAPA8, PPD1, zinc finger protein GLI1, GLI-Kruppel family member GLI1, glioma-associated oncogene 1, glioma-associated oncogene homolog 1 (zinc finger protein), oncogene GLI
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08151
  • Protein Name
    Zinc finger protein GLI1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Guo Q, Xuan MF, Luo ZB, et al. Baicalin improves the in vitro developmental capacity of pig embryos by inhibiting apoptosis, regulating mitochondrial activity and activating sonic hedgehog signaling. Mol Hum Reprod. 2019,25(9):538-549. doi: 10.1093/molehr/gaz036
    Read this paper
  • Chen X, Li XF, Chen Y, et al. Hesperetin derivative attenuates CCl(4)-induced hepatic fibrosis and inflammation by Gli-1-dependent mechanisms. Int Immunopharmacol. 2019,76:105838. doi: 10.1016/j.intimp.2019.105838
    Read this paper
  • Sato Y, Yamamura M, Sasaki M, et al. Blockade of Hedgehog Signaling Attenuates Biliary Cystogenesis in the Polycystic Kidney (PCK) Rat. Am J Pathol. 2018,188(10):2251-2263. doi: 10.1016/j.ajpath.2018.06.014
    Read this paper
  • Yang H, Wu S, Feng R, et al. Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats. Stem Cell Res Ther. 2017,8(1):267. doi: 10.1186/s13287-017-0718-8
    Read this paper
  • Fan H, Li H, Liu G, et al. Doxorubicin combined with low intensity ultrasound suppresses the growth of oral squamous cell carcinoma in culture and in xenografts. J Exp Clin Cancer Res. 2017,36(1):163. doi: 10.1186/s13046-017-0633-y
    Read this paper
  • Men Y, Zhang A, Li H, et al. LKB1 Regulates Cerebellar Development by Controlling Sonic Hedgehog-mediated Granule Cell Precursor Proliferation and Granule Cell Migration. Sci Rep. 2015,5:16232. doi: 10.1038/srep16232
    Read this paper
  • Liu X, Wang X, Du W, et al. Suppressor of fused (Sufu) represses Gli1 transcription and nuclear accumulation, inhibits glioma cell proliferation, invasion and vasculogenic mimicry, improving glioma chemo-sensitivity and prognosis. Oncotarget. 2014,5(22):11681-94.
    Read this paper
  • Du W, Liu X, Chen L, et al. Targeting the SMO oncogene by miR-326 inhibits glioma biological behaviors and stemness. Neuro Oncol. 2015,17(2):243-53. doi: 10.1093/neuonc/nou217
    Read this paper

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