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IHC-P analysis of human endometrium tissue using GTX83627 GLUT5 antibody [14C8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
IHC-P analysis of human endometrium tissue using GTX83627 GLUT5 antibody [14C8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
IHC-P analysis of human endometrium tissue using GTX83627 GLUT5 antibody [14C8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.

GLUT5 antibody [14C8]

GTX83627
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSLC2A5
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Overview

  • Supplier
    GeneTex
  • Product Name
    GLUT5 antibody [14C8] - Overexpression Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:2000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    14C8
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6518
  • Target name
    SLC2A5
  • Target description
    solute carrier family 2 member 5
  • Target synonyms
    glucose transporter type 5, small intestine; glucose transporter-like protein 5; GLUT5; GLUT-5; solute carrier family 2 (facilitated glucose/fructose transporter), member 5; solute carrier family 2, facilitated glucose transporter member 5; testicular tissue protein Li 81
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP22732
  • Protein Name
    Solute carrier family 2, facilitated glucose transporter member 5
  • Scientific Description
    Cytochalasin B-sensitive carrier. Seems to function primarily as a fructose transporter.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC2A5 using anti-SLC2A5 antibody (PB9960). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A5 antigen affinity purified polyclonal antibody (Catalog # PB9960) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A5 at approximately 55KD. The expected band size for SLC2A5 is at 55KD.
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