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Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA874819ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA874819ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA874819ESR1HU at dilution of 1:100

GNL3 Antibody

CSB-PA874819ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGNL3
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Overview

  • Supplier
    Cusabio
  • Product Name
    GNL3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26354
  • Target name
    GNL3
  • Target description
    G protein nucleolar 3
  • Target synonyms
    C77032, E2IG3, NNP47, NS, Nug1, guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, estradiol-induced nucleotide binding protein, guanine nucleotide binding protein-like 3 (nucleolar), novel nucleolar protein 47, nucleolar GTP-binding protein 3, nucleostemin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BVP2
  • Protein Name
    Guanine nucleotide-binding protein-like 3
  • Scientific Description
    May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Nucleostemin/GNL3 using anti-Nucleostemin/GNL3 antibody (A03283-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nucleostemin/GNL3 antigen affinity purified polyclonal antibody (Catalog # A03283-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nucleostemin/GNL3 at approximately 65 kDa. The expected band size for Nucleostemin/GNL3 is at 65 kDa.
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