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ProductsBack/Goat anti-CLLD8 / SETDB2 Antibody

Goat anti-CLLD8 / SETDB2 Antibody

EB05724
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetSETDB2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-CLLD8 / SETDB2 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID83852
  • Target name
    SETDB2
  • Target description
    SET domain bifurcated histone lysine methyltransferase 2
  • Target synonyms
    C13orf4, CLLD8, CLLL8, KMT1F, histone-lysine N-methyltransferase SETDB2, SET domain bifurcated 2, chronic lymphocytic leukemia deletion region gene 8 protein, lysine N-methyltransferase 1F
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_114121.2; NP_001153780.1. Purification: Antigen affinity purified. Names and symbols: SETDB2; CLLD8; SET domain, bifurcated 2; CLLL8 protein; C13orf4; CLLL8; DKFZp586I0123; DKFZp761J1217; KMT1F; chronic lymphocytic leukemia deletion region 8; lysine N-methyltransferase 1F; histone-lysine N-methyltransferase SETDB2
  • Reactivity
    Bovine, Canine, Human, Mouse, Porcine, Rat
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SETDB2 using anti-SETDB2 antibody (A08290-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SETDB2 antigen affinity purified polyclonal antibody (Catalog # A08290-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SETDB2 at approximately 82KD. The expected band size for SETDB2 is at 82KD.
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