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ProductsBack/SETDB2 antibody [N1], N-term
SETDB2 antibody [N1], N-term detects SETDB2 protein at nucleus by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SETDB2 protein stained by SETDB2 antibody [N1], N-term (GTX108086) diluted at 1:2000. Blue: Hoechst 33342 staining.
SETDB2 antibody [N1], N-term detects SETDB2 protein at nucleus by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SETDB2 protein stained by SETDB2 antibody [N1], N-term (GTX108086) diluted at 1:2000. Blue: Hoechst 33342 staining.
SETDB2 antibody [N1], N-term detects SETDB2 protein at nucleus by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SETDB2 protein stained by SETDB2 antibody [N1], N-term (GTX108086) diluted at 1:2000. Blue: Hoechst 33342 staining.

SETDB2 antibody [N1], N-term

GTX108086
GeneTex
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSETDB2
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Overview

  • Supplier
    GeneTex
  • Product Name
    SETDB2 antibody [N1], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    ICC/IF: 1:100-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID83852
  • Target name
    SETDB2
  • Target description
    SET domain bifurcated histone lysine methyltransferase 2
  • Target synonyms
    C13orf4, CLLD8, CLLL8, KMT1F, histone-lysine N-methyltransferase SETDB2, SET domain bifurcated 2, chronic lymphocytic leukemia deletion region gene 8 protein, lysine N-methyltransferase 1F
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96T68
  • Protein Name
    Histone-lysine N-methyltransferase SETDB2
  • Scientific Description
    Proteins that contain a SET domain, such as SETDB2, modulate gene expression epigenetically through histone H3 (see MIM 601128) methylation. SETDB2 is likely a histone H3 methyltransferase, as it contains both the active site and flanking cysteine residues required for catalytic activity (Zhang et al., 2003 [PubMed 12754510]).[supplied by OMIM]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SETDB2 using anti-SETDB2 antibody (A08290-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SETDB2 antigen affinity purified polyclonal antibody (Catalog # A08290-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SETDB2 at approximately 82KD. The expected band size for SETDB2 is at 82KD.
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