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SETDB2 Antibody

CAB7391
Assay Genie
ReactivityHuman
Product group Antibodies
TargetSETDB2
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Overview

  • Supplier
    Assay Genie
  • Product Name
    SETDB2 Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID83852
  • Target name
    SETDB2
  • Target description
    SET domain bifurcated histone lysine methyltransferase 2
  • Target synonyms
    C13orf4, CLLD8, CLLL8, KMT1F, histone-lysine N-methyltransferase SETDB2, SET domain bifurcated 2, chronic lymphocytic leukemia deletion region gene 8 protein, lysine N-methyltransferase 1F
  • Host
    Rabbit
  • Protein IDQ96T68
  • Protein Name
    Histone-lysine N-methyltransferase SETDB2
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SETDB2 using anti-SETDB2 antibody (A08290-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SETDB2 antigen affinity purified polyclonal antibody (Catalog # A08290-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SETDB2 at approximately 82KD. The expected band size for SETDB2 is at 82KD.
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Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CLLD8 Polyclonal Antibody, Unconjugated (bs-6414R) at 1:200 overnight at 4°C, DAB staining.
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