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Goat anti-CPEB1

EB06391
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCPEB1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-CPEB1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID64506
  • Target name
    CPEB1
  • Target description
    cytoplasmic polyadenylation element binding protein 1
  • Target synonyms
    CPEB; CPEB-1; CPE-binding protein 1; CPE-BP1; cytoplasmic polyadenylation element-binding protein 1; h-CPEB; hCPEB-1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_085097.3; NP_001073001.1; NP_001073002.1; NP_001073003.1. Purification: Antigen affinity purified. Names and symbols: CPEB1; CPEB; FLJ13203; cytoplasmic polyadenylation element binding protein 1; CEBP; CPE-BP1; MGC34136; MGC60106; CPE-binding protein 1
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CPEB1 using anti-CPEB1 antibody (M03578). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPEB1 antigen affinity purified monoclonal antibody (Catalog # M03578) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPEB1 at approximately 70 kDa. The expected band size for CPEB1 is at 63 kDa.
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