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ProductsBack/Goat anti-EP4 prostaglandin receptor

Goat anti-EP4 prostaglandin receptor

EB07123
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPTGER4
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-EP4 prostaglandin receptor Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5734
  • Target name
    PTGER4
  • Target description
    prostaglandin E receptor 4
  • Target synonyms
    EP4; EP4R; PGE receptor, EP4 subtype; PGE2 receptor EP4 subtype; prostaglandin E receptor 4 (subtype EP4); prostaglandin E2 receptor EP4 subtype; prostanoid EP4 receptor
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000949.1. Purification: Antigen affinity purified. Names and symbols: prostaglandin E receptor 4 (subtype EP4) ; MGC126583 ; PTGER4; EP4; EP4R; PGE receptor, EP4 subtype prostaglandin E receptor 4, subtype EP4; prostaglandin E2 receptor
  • Reactivity Supplier
    Human, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PTGER4 using anti-PTGER4 antibody (A02153-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: SW620 whole Cell lysates, Lane 2: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGER4 antigen affinity purified polyclonal antibody (Catalog # A02153-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGER4 at approximately 53KD. The expected band size for PTGER4 is at 53KD.
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