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Western Blot analysis of COLO205 cells using EP4 Polyclonal Antibody
Western Blot analysis of COLO205 cells using EP4 Polyclonal Antibody
Western Blot analysis of COLO205 cells using EP4 Polyclonal Antibody

PTGER4 Antibody

CSB-PA002354
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPTGER4
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Overview

  • Supplier
    Cusabio
  • Product Name
    PTGER4 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5734
  • Target name
    PTGER4
  • Target description
    prostaglandin E receptor 4
  • Target synonyms
    EP4, EP4R, prostaglandin E2 receptor EP4 subtype, PGE receptor, EP4 subtype, PGE2 receptor EP4 subtype, prostaglandin E receptor 4 (subtype EP4), prostanoid EP4 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35408
  • Protein Name
    Prostaglandin E2 receptor EP4 subtype
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PTGER4 using anti-PTGER4 antibody (A02153-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: SW620 whole Cell lysates, Lane 2: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGER4 antigen affinity purified polyclonal antibody (Catalog # A02153-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGER4 at approximately 53KD. The expected band size for PTGER4 is at 53KD.
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