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ProductsBack/Goat anti-Furin (aa553-565)

Goat anti-Furin (aa553-565)

EB11525
Everest Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetFURIN
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Furin (aa553-565) Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5045
  • Target name
    FURIN
  • Target description
    furin, paired basic amino acid cleaving enzyme
  • Target synonyms
    dibasic processing enzyme; FES upstream region; FUR; furin; furin, membrane associated receptor protein; PACE; paired basic amino acid residue-cleaving enzyme; PCSK3; proprotein convertase subtilisin/kexin 3; proprotein convertase subtilisin/kexin type 3; SPC1; subtilisin-like proprotein convertase 1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002560.1. GeneIDs all Nonhuman: 18550 (mouse); 54281 (rat);. Purification: Antigen affinity purified. Names and symbols: FURIN; furin (paired basic amino acid cleaving enzyme); FUR; PACE; PCSK3; SPC1; FES upstream region; OTTHUMP00000194747; dibasic processing enzyme; dibasic-processing enzyme; furin; furin, membrane associated receptor protein; paired basic amino acid resi
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Furin using anti-Furin antibody (PB9182). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human Furin protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Furin antigen affinity purified polyclonal antibody (Catalog # PB9182) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Furin at approximately 40 kDa. The expected band size for Furin is at 40 kDa.
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