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Goat anti-GSTM1 / GSTM2

EB06606
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetGSTM1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-GSTM1 / GSTM2 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID2944
  • Target name
    GSTM1
  • Target description
    glutathione S-transferase mu 1
  • Target synonyms
    glutathione S-alkyltransferase; glutathione S-aralkyltransferase; glutathione S-aryltransferase; glutathione S-transferase M1; glutathione S-transferase Mu 1; GST class-mu 1; GST HB subunit 4; GST1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4; GTM1; H-B; HB subunit 4; MU; MU-1; S-(hydroxyalkyl)glutathione lyase
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000552.2; NP_666533.1; NP_000839.1. Purification: Antigen affinity purified. Names and symbols: GSTM1; GSTM2; MU; H-B; GST1; GTH4; GTM1; MU-1; GSTM1-1; MGC26563; GSTM1a-1a; GSTM1b-1b; glutathione S-transferase M1; HB subunit 4; GST class-mu 1; glutathione S-aryltransferase; glutathione S-alkyltransferase; glutathione S-transferase, Mu-1; glutathione
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Inhibition of aryl hydrocarbon receptor transactivation and DNA adduct formation by CYP1 isoform-selective metabolic deactivation of benzo[a]pyrene. Endo K et al., 2008 Jul 15, Toxicol Appl Pharmacol
    Read more

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Figure 1. Western blot analysis of GSTM1 using anti-GSTM1 antibody (A00569-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse stomach tissue lysates, Lane 4: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTM1 antigen affinity purified polyclonal antibody (Catalog # A00569-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSTM1 at approximately 26KD. The expected band size for GSTM1 is at 26KD.
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