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Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA009979LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA009979LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA009979LA01HU at dilution of 1:100

GSTM1 Antibody

CSB-PA009979LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGSTM1
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Overview

  • Supplier
    Cusabio
  • Product Name
    GSTM1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2944
  • Target name
    GSTM1
  • Target description
    glutathione S-transferase mu 1
  • Target synonyms
    glutathione S-alkyltransferase; glutathione S-aralkyltransferase; glutathione S-aryltransferase; glutathione S-transferase M1; glutathione S-transferase Mu 1; GST class-mu 1; GST HB subunit 4; GST1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4; GTM1; H-B; HB subunit 4; MU; MU-1; S-(hydroxyalkyl)glutathione lyase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09488
  • Protein Name
    Glutathione S-transferase Mu 1
  • Scientific Description
    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GSTM1 using anti-GSTM1 antibody (A00569-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse stomach tissue lysates, Lane 4: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTM1 antigen affinity purified polyclonal antibody (Catalog # A00569-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSTM1 at approximately 26KD. The expected band size for GSTM1 is at 26KD.
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